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Genome Sequence of Halomonas sp. Strain MCTG39a, a Hydrocarbon-Degrading and Exopolymeric Substance-Producing Bacterium

Authors

Tony Gutierrez, William B. Whitman, Marcel Huntemann, Alex Copeland, Amy Chen, Nikos Kyrpides, Victor Markowitz, Manoj Pillay, Natalia Ivanova, Natalia Mikhailova, Galina Ovchinnikova, Evan Andersen, Amrita Pati, Dimitrios Stamatis, T. B. K. Reddy, Chew Yee Ngan, Mansi Chovatia, Chris Daum, Nicole Shapiro, Michael N. Cantor, Tanja Woyke

Abstract

Halomonas sp. strain MCTG39a was isolated from coastal sea […]

11 July 2016|Tags: , |

Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill

Authors

Tony Gutierrez, David Berry, Tingting Yang, Sara Mishamandani, Luke McKay, Andreas Teske, Michael D. Aitken

Abstract

Halomonas species are recognized for producing exopolysaccharides (EPS) exhibiting amphiphilic properties that allow these macromolecules to interface with hydrophobic substrates, such as hydrocarbons. There remains a paucity of knowledge, however, on the potential of Halomonas EPS to influence the […]

11 July 2016|Tags: , , |

Glycoprotein emulsifiers from two marine Halomonas species: chemical and physical characterization

Authors

T. Gutierrez, B. Mulloy, K. Black and D. H. Green

Abstract

Aims: To partially purify and characterize bioemulsifiers produced by two new marine Halomonas species, TG39 and TG67, and to compare their emulsifying activities with those of commercial emulsifiers. Methods and Results: The production of emulsifiers HE39 and HE67 was achieved from glucose-supplemented marine broth, and recovered […]

Yield and Physicochemical Properties of EPS From Halomonas sp. Strain TG39 Identifies a Role for Protein and Anionic Residues (Sulfate and Phosphate) in Emulsification of n-Hexadecane

Authors

Tony Gutierrez, Gordon Morris, David H. Green

Abstract

In this study, we investigated the yield and physicochemical properties of the high molecular weight extracellular polymeric substance (HMW–EPS) produced by Halomonas sp. strain TG39 when grown on different types and ratios of substrates. Glucose (1% w/v) and a peptone/yeast extract ratio of 5.1 (0.6% w/v final concentration) yielded […]