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Surface-active biopolymers from marine bacteria for potential biotechnological applications

Authors

Karina Sałek and Tony Gutierrez

Abstract

Surface-active agents are amphiphilic chemicals that are used in almost every sector of modern industry, the bulk of which are produced by organo-chemical synthesis. Those produced from biological sources (biosurfactants and bioemulsifiers), however, have gained increasing interest in recent years due to their wide structural and functional diversity, lower […]

Genome Sequence of Halomonas sp. Strain MCTG39a, a Hydrocarbon-Degrading and Exopolymeric Substance-Producing Bacterium

Authors

Tony Gutierrez, William B. Whitman, Marcel Huntemann, Alex Copeland, Amy Chen, Nikos Kyrpides, Victor Markowitz, Manoj Pillay, Natalia Ivanova, Natalia Mikhailova, Galina Ovchinnikova, Evan Andersen, Amrita Pati, Dimitrios Stamatis, T. B. K. Reddy, Chew Yee Ngan, Mansi Chovatia, Chris Daum, Nicole Shapiro, Michael N. Cantor, Tanja Woyke

Abstract

Halomonas sp. strain MCTG39a was isolated from coastal sea […]

11 July 2016|Tags: , |

Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill

Authors

Tony Gutierrez, David Berry, Tingting Yang, Sara Mishamandani, Luke McKay, Andreas Teske, Michael D. Aitken

Abstract

Halomonas species are recognized for producing exopolysaccharides (EPS) exhibiting amphiphilic properties that allow these macromolecules to interface with hydrophobic substrates, such as hydrocarbons. There remains a paucity of knowledge, however, on the potential of Halomonas EPS to influence the […]

11 July 2016|Tags: , , |

Metal binding properties of the EPS produced by Halomonas sp. TG39 and its potential in enhancing trace element bioavailability to eukaryotic phytoplankton

Authors

Tony Gutierrez, Dondra V. Biller, Tracy Shimmield, David H. Green

Abstract

An emergent property of exopolysaccharides (EPS) produced by marine bacteria is their net negative charge, predominantly conferred by their high uronic acids content. Here, we investigated the EPS produced by an algal-associated marine bacterium, Halomonas sp. strain TG39, for its capacity to sequester trace […]

Emulsifying properties of a glycoprotein extract produced by a marine Flexibacter species strain TG382

Authors

Tony Gutiérreza, Vincent V. Leob, Graeme M.Walkerb, David H. Green

Abstract

We report, for the first time, on the production of an emulsifying polymer produced by a Flexibacter species (designated strain TG382). This polymer, E-382,was produced extracellularly during growth of the organism in a marine broth amended with glucose. After cold ethanol precipitation, extensive dialysis […]

Glycoprotein emulsifiers from two marine Halomonas species: chemical and physical characterization

Authors

T. Gutierrez, B. Mulloy, K. Black and D. H. Green

Abstract

Aims: To partially purify and characterize bioemulsifiers produced by two new marine Halomonas species, TG39 and TG67, and to compare their emulsifying activities with those of commercial emulsifiers. Methods and Results: The production of emulsifiers HE39 and HE67 was achieved from glucose-supplemented marine broth, and recovered […]

Isolation of glycoprotein bioemulsifiers produced by marine bacteria. In Hydrocarbon and Lipid Microbiology Protocols, Springer Protocols Handbooks, Eds. McGenity et al. DOI 10.1007/8623_2014_1, Springer-Verlag Berlin Heidelberg. (In press)

Authors

Tony Gutierrez, Ibrahim M. Banat

Abstract

High-molecular-weight (HMW) surface-active agents (biosurfactants and bioemulsifiers) are produced by many different types of bacteria and comprise proteins, glycoproteins, lipoproteins, polysaccharides, lipopolysaccharides or complexes containing any combination of these structural types. The attraction of HMW glycoprotein bioemulsifiers as ingredients in food and drink formulations, for example, has increased […]

18 November 2015|Tags: , , , |

Yield and Physicochemical Properties of EPS From Halomonas sp. Strain TG39 Identifies a Role for Protein and Anionic Residues (Sulfate and Phosphate) in Emulsification of n-Hexadecane

Authors

Tony Gutierrez, Gordon Morris, David H. Green

Abstract

In this study, we investigated the yield and physicochemical properties of the high molecular weight extracellular polymeric substance (HMW–EPS) produced by Halomonas sp. strain TG39 when grown on different types and ratios of substrates. Glucose (1% w/v) and a peptone/yeast extract ratio of 5.1 (0.6% w/v final concentration) yielded […]

Emulsifying and Metal Ion Binding Activity of a Glycoprotein Exopolymer Produced by Pseudoalteromonas sp. Strain TG12

Authors

Tony Gutierrez, Tracy Shimmield, Cheryl Haidon, Kenny Black, and David H. Green

Abstract

In this study, we describe the isolation and characterization of a new exopolymer that exhibits high emulsifying activities against a range of oil substrates and demonstrates a differential capacity to desorb various mono-, di-, and trivalent metal species from marine sediment under nonionic and […]

6 November 2015|Tags: , |

Partial purification and chemical characterization of a glycoprotein (putative hydrocolloid) emulsifier produced by a marine bacterium Antarctobacter

Authors

Tony Gutiérrez, Barbara Mulloy, Charlie Bavington, Kenny Black, David H. Green

Abstract

During screening for novel emulsifiers and surfactants, a marine alphaproteobacterium, Antarctobacter sp. TG22, was isolated and selected for its production of an extracellular emulsifying agent, AE22. This emulsifier was produced optimally in a low-nutrient seawater medium supplemented with glucose and was extractable by cold ethanol […]

12 October 2015|Tags: , , , |