Funded by the European Union Framework Programme for research and innovation, Horizon 2020 under grant agreement No 635340|info@marisurf.eu
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Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill

Authors

Tony Gutierrez, David Berry, Tingting Yang, Sara Mishamandani, Luke McKay, Andreas Teske, Michael D. Aitken

Abstract

Halomonas species are recognized for producing exopolysaccharides (EPS) exhibiting amphiphilic properties that allow these macromolecules to interface with hydrophobic substrates, such as hydrocarbons. There remains a paucity of knowledge, however, on the potential of Halomonas EPS to influence the […]

11 July 2016|Tags: , , |
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Metal binding properties of the EPS produced by Halomonas sp. TG39 and its potential in enhancing trace element bioavailability to eukaryotic phytoplankton

Authors

Tony Gutierrez, Dondra V. Biller, Tracy Shimmield, David H. Green

Abstract

An emergent property of exopolysaccharides (EPS) produced by marine bacteria is their net negative charge, predominantly conferred by their high uronic acids content. Here, we investigated the EPS produced by an algal-associated marine bacterium, Halomonas sp. strain TG39, for its capacity to sequester trace […]

11 July 2016|Tags: , , , , , |
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Emulsifying properties of a glycoprotein extract produced by a marine Flexibacter species strain TG382

Authors

Tony Gutiérreza, Vincent V. Leob, Graeme M.Walkerb, David H. Green

Abstract

We report, for the first time, on the production of an emulsifying polymer produced by a Flexibacter species (designated strain TG382). This polymer, E-382,was produced extracellularly during growth of the organism in a marine broth amended with glucose. After cold ethanol precipitation, extensive dialysis […]

11 July 2016|Tags: , , , , , |
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Glycoprotein emulsifiers from two marine Halomonas species: chemical and physical characterization

Authors

T. Gutierrez, B. Mulloy, K. Black and D. H. Green

Abstract

Aims: To partially purify and characterize bioemulsifiers produced by two new marine Halomonas species, TG39 and TG67, and to compare their emulsifying activities with those of commercial emulsifiers. Methods and Results: The production of emulsifiers HE39 and HE67 was achieved from glucose-supplemented marine broth, and recovered […]

11 July 2016|Tags: , , , , , |
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Yield and Physicochemical Properties of EPS From Halomonas sp. Strain TG39 Identifies a Role for Protein and Anionic Residues (Sulfate and Phosphate) in Emulsification of n-Hexadecane

Authors

Tony Gutierrez, Gordon Morris, David H. Green

Abstract

In this study, we investigated the yield and physicochemical properties of the high molecular weight extracellular polymeric substance (HMW–EPS) produced by Halomonas sp. strain TG39 when grown on different types and ratios of substrates. Glucose (1% w/v) and a peptone/yeast extract ratio of 5.1 (0.6% w/v final concentration) yielded […]

6 November 2015|Tags: , , , , , , |
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